Panexin basic Panexin basic is a chemically defined serum replacement for the cultivation of adherent and non-adherent cells under serum-free culture conditions or to significantly reduce the amount of serum in cell culture. It supports the growth of many cell types in an optimum manner without any extra handling compared to serum. Description Size Product number Datasheet Panexin basic 50 ml100 ml500 ml P04-96090P04-96900P04-96950    Composition Panexin basic contains purified proteins, lipids, salts, amino acids, trace elements, hormones and a 3-dimensional substrate release system in an optimized formulation. It contains no growth factors, undefined hydrolysates or peptones.    Suitability Panexin basic is suitable for the cultivation of a variety of adherent and non-adherent cells under serum-free culture conditions. Further informations can be found in the datasheet.    Special advantages Panexin basic is designed to replace or to reduce serum in the cell culture in a very simple manner. In most cases there is no need to change the basal medium. As Panexin basic is fully chemically defined and contains no peptones or hydrolysates, lot testing is no more necessary. It also allows high reproducibility and a simplified downstream process. Panexin basic contains no growth factors and enables defined proliferation and differentiation of stem cells. Characterization studies of growth factors will obtain more reproducible and clearer results. Panexin basic is also useful to develop sensitive cell-based in vitro tests and coculture procedures.    References by cell lines Panexin products are chemically defined serum substitutes, which have been successfully applied for a wide range of cell cultures.      Tumor Cells    Human pancreatic adenocarcinoma cells • Gamper et al., Cell Biology International Volume 40, Issue 10, Pages 1050–106, 2016.  http://onlinelibrary.wiley.com/doi/10.1002/cbin.10645/full  • Freud et al., Preprints 2016, 2016100056   www.preprints.org/manuscript/201610.0056/v1/download  • Schlingensiepen et al., Cancer Science Volume 102, Issue 6 June 2011 Pages 1193–1200  http://onlinelibrary.wiley.com/doi/10.1111/j.1349-7006.2011.01917.x/full  • HouHou et al., Patent US20110171213 A1 Human prostate cancer cells  • Lisa Rauschenberger, Dissertation „Strukturelle und funktionelle Charakterisierung von Exosomen aus Prostatakarzinomzellen“, 2016.  http://d-nb.info/111106475X/34  • Rauschenberger et al., Prostate. 2016 Mar;76(4):409-24  https://www.ncbi.nlm.nih.gov/pubmed/26643154  • Stope et al., Anticancer Res. 2013 Nov;33(11):4921-6  https://www.ncbi.nlm.nih.gov/pubmed/24222130  Human liposarcoma  • Harati et al., Int J Mol Med. 2016 Jun; 37(6): 1535–1541.  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4867885/pdf/ijmm-37-06-1535.pdf  Tumor (adenocarcinoma) primary fibroblasts  • Castoldi, Dissertation “Generation and characterization of multi-specific antibodies for therapeutic applications in oncology”, 2014.  https://edoc.ub.uni-muenchen.de/16625/1/Castoldi_Raffaella.pdf  Human hepatoblastoma cells  • S Beckers, Dissertation “High throughput toxicity, physiological and metabolic studies for the characterization of hepatocytes and human embryonic stem cell derived hepatocyte-like cells.”, 2011 http://scidok.sulb.uni-saarland.de/volltexte/2011/3665/pdf/Dissertation_Simone_Beckers_23.03_neu.pdf  Human breast cancer cell  • Peter et al., Journal of Biomolecular Techniques 18:287–297 © 2007 ABRF  https://www.researchgate.net/profile/Jochen_Peter/publication/5682413_Enrichment_and_detection_of_molecules_secreted_by_tumor_cells_using_magnetic_reversed-phase_particles_and_LC-MALDI-TOF-MS/links/5460abd40cf2c1a63bfeb17e.pdf     Other Human Cells   Rheumatoid arthritis synovial fibroblasts  • Zimmermann-Geller et al., The Journal of Immunology October 1, 2016 vol. 197 no. 7 2589-2597.  http://www.jimmunol.org/content/197/7/2589.short  Coculture: Human liposarcoma cells and tumor-associated fibroblasts  • Harati et al., Int J Mol Med. 2016 Jun; 37(6): 1535–1541.  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4867885/pdf/ijmm-37-06-1535.pdf.  Coculture: MRC5 and tumor-associated fibroblasts  • Majety et al., PLOS ONE | DOI:10.1371/journal.pone.0127948 June 8, 2015  http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0127948&type=printable.  Coculture: SZ95 sebocytes and normal human fibroblasts  • Nikolakis et al., https://www.ncbi.nlm.nih.gov/pubmed/25828468  • http://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0127948&type=printable.  Coculture: H596 and normal and tumor (adenocarcinoma) primary fibroblasts  • Castoldi, Dissertation “Generation and characterization of multi-specific antibodies for therapeutic applications in oncology”, 2014  https://edoc.ub.uni-muenchen.de/16625/1/Castoldi_Raffaella.pdf  Human corneal epithelial cells  • Hahne et al., Int J Pharm. 2011 Sep 15;416(1):268-79  https://www.ncbi.nlm.nih.gov/pubmed/21771646  Human myometrial microvascular endothelial cells  • Dietrich et al., Fertil Steril. 2011 Mar 15;95(4):1247-55.e1-2  https://www.ncbi.nlm.nih.gov/pubmed/21130428     Stem Cells  rMSC  • Puts et al., Ultrasound in Medicine & Biology, Volume 42, Issue 12, December 2016, Pages 2965–2974.  http://ieeexplore.ieee.org/document/7317777/  • Puts et al., Ultrasonics Symposium (IUS), 2014 IEEE International,3-6 Sept. 2014  http://ieeexplore.ieee.org/document/6932303/  hMSC  • Ichikawa et al., Cell Biol Int. 2010 Apr 27;34(6):615-20  https://www.ncbi.nlm.nih.gov/pubmed/20218971  Hematopoietic cells  • Ridder et al., PLoS Biol. 2014 Jun; 12(6): e1001874.  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4043485/     Other Cell Lines  Bone marrow derived macrophages  • Stolt et al., J Immunol. 2016 Aug 1;197(3):834-46.  http://www.jimmunol.org/content/197/3/834.short  • Hommes et al., Am J Respir Cell Mol Biol. 2015 Nov;53(5):647-55  http://www.atsjournals.org/doi/pdf/10.1165/rcmb.2014-0485OC  • Hof et al., Infect Immun. 2014 May; 82(5): 2006–2015  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3993457/  • Depke et al., Journal of Proteomics Volume 103, 30 May 2014, Pages 72–86  http://www.sciencedirect.com/science/article/pii/S1874391914001493  • Schramm et al., Eur. J. Immunol. 2014. 44: 728–741.  http://onlinelibrary.wiley.com/doi/10.1002/eji.201343940/full  • Bast et al., LoS Pathog 10(3): e1003986. doi:10.1371/journal.ppat.1003986.  http://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.1003986  • Koh et al., PLoS Negl Trop Dis. 2013 Oct; 7(10): e2500  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3798430/  • Farinacci et al., Vaccine. 2012 Dec 14;30(52):7608-14  https://www.ncbi.nlm.nih.gov/pubmed/23088886  • Erttmann et al., Free Radic Biol Med. 2011 Aug 1;51(3):626-40  http://www.sciencedirect.com/science/article/pii/S0891584911003248  • Norville et al., Microbiology. 2011 Sep;157(Pt 9):2629-38  https://www.ncbi.nlm.nih.gov/pubmed/21680634  • Breitbach et al., BMC Immunol. 2011; 12: 20  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3072354/  • Bast et al., Toxicol In Vitro. 2010 Mar;24(2):686-94.  https://www.ncbi.nlm.nih.gov/pubmed/20869433  • Eske et al., J Immunol Methods. 2009 Mar 15;342(1-2):13-9.  https://www.ncbi.nlm.nih.gov/pubmed/19133267  • Breitbach et al., Infect Immun. 2009 Apr; 77(4): 1589–1595  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2663179/  • Traeger et al., Infect Immun. 2008 Nov; 76(11): 5285–5293  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2573314/   HEK   • Lisa Rauschenberger, Dissertation „Strukturelle und funktionelle Charakterisierung von Exosomen aus Prostatakarzinomzellen“, 2016.  http://d-nb.info/111106475X/34  • Into et al., Mol Cell Biol. 2008 Feb; 28(4): 1338–1347.  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2258749/   And many more!!!