SiCORE   SiCORE are 1 µm sized non-magnetic, monodisperse microspheres based on a silica matrix. The advantage of silica microspheres compared to polystyrene microspheres are the inert and stable solid-phase silica matrix without autofluorescence and are autoclaved for sterile applications. Furthermore through the higher mechanical and chemical stability, hydrophilic character, resistance to microbiological degradation and compatible with organic solvents is it useful for biochromatiography applications.   SiCORE - active   SiCORE-active-microspheres are useful for covalent binding of bioaffine ligands, such as proteins or antibodies, as a tool for diagnostic application, e.g. Agglutination Test (LAT), Lateral flow assays or Particle Capture ELISA. See: Coupling-Protocols   Application: Activated particles for custom-made covalent binding of biological substances Formulation: suspension in ddH2O, autoclaved Concentration: 50 mg/ml Particle size: 1.0 µm       Functional Group Product Number Quantity (ml) Activation / Coupling Product Information Reference Number SiCORE-Amine -O-(CH2)3-NH2 6401-16401-5 15 Carbodiimide method Mannich reaction see coupling protocol: C2/3 - SiCORE-Carboxyl -O-(CH2)3-COOH 6402-16402-5 15 Carbodiimide method see coupling protocol: C1 - SiCORE-Hydrazide -O-(CH2)3~CO-NH-NH2 6404-16404-5 15 Oxidized carbohydrates see coupling protocol: C5 - SiCORE-Silanol -Si-OH 6408-16408-5 15 Binding of DNA/RNA, adsorption of biomolecules - SiCORE-Hydroxyl -Si-(CH2)3-OH 6405-16405-5 15 Cyanogen bromide activation see coupling protocol: A7 - SiCORE-PGL -Si-(CH2)3 6406-16406-5 15 For covalent coupling of bioligands see protocol: A4 - SiCORE-Thiol -Si-(CH2)3-SH 6407-16407-5 15 Maleimides see coupling protocol: A6 -   SiCORE - affinity   SiCORE-affinity - bioaffine ligands are covalently bound to the microspheres. High selectivity and high capacity make these products for an ideal tool for the binding of specific biomolecules. See: Coupling-Protocols   Application: Affinity chromatography Formulation: buffer suspension, 0.01 NaN3 Concentration: 10 mg/ml Particle sizes: 1.0 µm     Affinity Group Product Number Quantity (ml) For Purification Product Information Reference Number SiCORE-Biotin 6503-16503-5 15 Avidin / streptavidin labeled molecules - SiCORE-Boronic acid 6504-16504-5 15 Ribonucleotides, sugars via cis-diols see protocol: CB14 - SiCORE-Concanavalin A 6506-16506-5 15 Bacteria, glycoproteins - SiCORE-Goat anti-rabbit IgG(whole molecule) 6520-16520-5 15 For binding of secondary antibody See protocol: CB11 - SiCORE-Goat anti-mouse IgG(whole molecule) 6510-16510-5 15 For binding of secondary antibody See protocol: CB4 - SiCORE-Protein A 6515-16515-5 15 Antibodies See protocol: CB2 - SiCORE-Protein G 6516-16516-5 15 - SiCORE-Streptavidin 6517-16517-5 15 Biotin-labeled molecules See protocol: CB1 -