fluidMAG fluidMAG-nanoparticles are ferrofluids consisting of an aqueous dispersion of magnetic iron oxides with diameters of 50 nm, 100 nm and 200 nm. The particles are covered with hydrophilic polymers which protect them against aggregation by foreign ions. Terminal functional groups such as ion-exchange groups or reactive groups for covalent immobilization can be used for binding to biomolecules. Ferrofluids can further be used for MRI-diagnostics and magnetic drug targeting applications. Application: Cell separation, MRI-diagnostics, Magnetic Drug-Targeting Formulation: suspension in ddH2O, autoclaved Concentration: 25 mg/ml Particle sizes: 50 nm, 100 nm, 200 nm; ( 75 nm, 150 nm on request) TEM-multi-domain magnetite core TEM-single-domain magnetite cores     fluidMAG-nanoparticles with hydrodynamic diameters of 100-200 nm have a multi-domain core and in-batch separable by a external magnetic field (magnetic separator). Therefore, a fast and easy magnetic isolation of nanoparticles is possible. Ferrofluids with particle sizes of 50 nm have a single domain core and therefore not separable by an external magnetic field. These substances are real magnetic fluids, and the magnetic isolation is only possible with a High Gradient Magnetic Separator (e.g. Miltenyi® separation columns). This is expensive and time consuming; therefore we recommend using magnetic nanoparticles with a minimum size of 100 nm.           Product Polymer Matrix Product Number Quantity (ml) Application Product Information Reference Number fluidMAG-Amine Amine silane 4121-14121-5 15 For covalent coupling of biomolecules 1.35, 1.48, 1.57,1.62, 3.09, 3.18,10.37 fluidMAG-ARA Glucuronic acid 4115-14115-5 15 Weak cation exchanger; for covalent coupling of biomolecules (antibodies or proteins) See protocol: A10 1.02, 1.13, 1.22,1.63, 1.68, 3.04,3.07, 3.14, 3.15,3.16, 3.17, 3.19,3.23, 4.05, 5.03,5.21, 5.22, 10.34 fluidMAG-Bromoacetyl**Available only in a hydrodynamic diameter of 200 nm. Bromoacetyl 4128-14128-5 15 For binding of SH-groups containing bioligands see coupling protocol: A14 - fluidMAG-Chitosan Chitosan 4118-14118-5 15 Linear, biodegradable polymer with amino-groups 3.20, 3.22, 6.11 fluidMAG-CMX Carboxymethyldextran 4106-14106-5 15 Weak cation-exchanger See protocol: A11 1.14, 3.18, 4.12,5.01, 5.10, 6.05,10.30 fluidMAG-CT Citric acid 4122-14122-5 15 For MRI diagnostic and for binding of cationic molecules 3.10 fluidMAG-D Starch 4101-14101-5 15 Isolation of lysosomes, MRI-diagnostic, for covalent coupling of biomolecules (antibodies or proteins) See protocol: A9 1.02, 1.03, 1.20,3.03, 3.13, 3.22,4.01, 4.02, 4.04,4.07, 4.10, 4.14,5.04, 5.11, 5.15,5.16, 5.17, 5.20,7.03, 7.04, 7.06,10.07, 10.15, 10.18,10.21, 10.22, 10.30,10.35, 10.37, 10.38,10.40, 10.42 fluidMAG-DEAE DEAE-starch 4102-14102-5 15 Binds negative groups of cells, bacteria, viruses 1.01, 2.07, 2.08,3.21, 4.02, 4.03,4.14, 5.02 fluidMAG-DP Phosphate-starch 4103-14103-5 15 Purification of basic proteins, drug targeting 1.01, 4.02, 4.03,8.01, 8.02, 8.03,8.04, 10.36 fluidMAG-DX Dextran 4104-14104-5 15 Activation by cyanogen bromide 1.37, 1.68, 4.03,5.10, 6.05, 7.05,7.07, 8.08 fluidMAG-DXS Dextran-sulfate 4105-14105-5 15 Strong cation-exchanger 5.10, 10.36 fluidMAG-Lipid Lipid 4119-14119-5 15 Negatively charged phosphatidylcholine 4.14 fluidMAG-OS Oleic acid 4113-14113-5 15 Hydrophilic / hydrophobe surface - fluidMAG-P Diphosphate 4123-14123-5 15 For binding of cationic molecules 4.14 fluidMAG-PAA Polyaspartic acid 4108-14108-5 15 Strong cation-exchanger 2.07, 10.36 fluidMAG-PAD Polyacrylamide 4109-14109-5 15 Surface modification 1.40 fluidMAG-PAS Polyacrylic acid 4110-14110-5 15 Weak cation-exchanger See protocol: A6 5.04, 5.07, 5.14,5.23 fluidMAG-PEA Polydimethylamine 4117-14117-5 15 Terminal amino groups for conjugation of biomolecules 1.29 fluidMAG-PEG/Amine Polyethylene glycol α-Methoxy-ω-amine 4131-14131-5 15 Aqueous dispersion of polyethylene glycol α-Methoxy-ω-amine covered magnetic nanoparticles - fluidMAG-PEG/P Polyethylene glycol α-, ω-diphosphate 4116-14116-5 15 Aqueous dispersion of polyethylene glycol α-, ω-diphosphate covered magnetic nanoparticles - fluidMAG-PMO Poly(maleic acid-co-olefin) 4124-14124-5 15 For hydrophilic / hydrophobe interaction - fluidMAG-PS Polystyrenesulfonate 4111-14111-5 15 Strong cation-exchanger 1.19 fluidMAG-PVA Polyvinyl alcohol 4120-14120-5 15   - fluidMAG-PVPY Poly(4-vinylpyridine) 4129-14129-5 15 For binding of enzymes - fluidMAG-Q Poly-diallyldimethylamin 4112-14112-5 15 Strong anion-exchanger 1.15, 1.17, 2.07,2.08, 4.14, 5.02,5.09, 5.10 fluidMAG-UC/A Magnetite uncoated 4114-14114-5 15 Adsorption of biomolecules stabilized by cross-linking; anionic charged 1.25, 1.27, 1.68,3.18, 10.48 fluidMAG-UC/C  Magnetite uncoated 4130-14130-5 15 Adsorption of biomolecules stabilized by cross-linking; cationic charged 1.68, 10.48     fluidMAG - affinity fluidMAG-affinity - bioaffine ligands are covalently bound to magnetic particles. High selectivity and high capacity make these products an ideal tool for the isolation of specific biomolecules. The 100 nm and 250 nm fluidMAG-affinity particles are in-batch separable by an external magnetic field (magnetic separator). Application: Affinity chromatography Formulation: buffer suspension, 0.01 NaN3 Concentration: 10 mg/ml Particle sizes: 100 nm, 200 nm   fluidMAG with Ligand Product Number Quantity (ml) Application for:Purification / Binding Product Information Reference Number fluidMAG-Biotin 4201-14201-5 15 Avidin / streptavidin labeled molecules - fluidMAG-Goat anti-mouse IgG(whole molecule) 4202-14202-5 15 Binding of secondary antibody See protocol: B4 3.11, 4.05 fluidMAG-Protein A 4203-14203-5 15 Antibodies See protocol: B2 1.48 fluidMAG-Protein G 4204-14204-5 15 1.48, 1.65 fluidMAG-Streptavidin 4205-14205-5 15 Biotin-labeled molecules See protocol: B1 1.33, 1.39, 1.48,1.51, 3.05, 4.06,5.08, 5.13, 8.06 fluidMAG-Casein 4206-14206-5 15 RNA polymerase - fluidMAG-Gelatin 4207-14207-5 15 Fibronectin - fluidMAG-Heparin 4208-14208-5 15 Lipoprotein, lipase, RNA-polymerase 8.05