screenMAG
screenMAG-particles are magnetic-fluorescent beads based on the well-known SiMAG technology. The advantage of both innovative techniques - the magnetic- and the fluorescence properties - provide exciting new possibilities to explore new fields of application in biosciences.
FluorescenceType
FluorescenceColor
Excitation
Emission
screenMAG/B
blue
400 nm
420 nm
screenMAG/G
green
502 nm
525 nm
screenMAG/O
orange
526 nm
555 nm
screenMAG/OP
orange
536 nm
617 nm
screenMAG/RR
red
540 nm
625 nm
screenMAG/R
red
633 nm
672 nm
Fluorescence image of screenMAG/G
particles under magnetic field
Fluorescence image of screenMAG/O
particles under magnetic field
NOTE: The fluorescence of the screenMAG particles is only detectable on the same side where the excitation takes place.
Please note that there is a difference in fluorescence observation between dissolved fluorescence molecules and solid fluorescence particles. Fluorescence spectrophotometer with a fluorescence detection unit with an angle of 90° to the excitation source will detect no or only weak fluorescence signals.
screenMAG-active
screenMAG-active - terminal reactive groups of the magnetic-fluorescent beads allow the convenient and efficient covalent binding of bioaffine ligands. This is a unique feature accounting for the rapidly increasing number and variation of novel and different receptor-affine biological substances.
Application:
Magnetic separation and fluorescence identification of targets
Formulation:
suspension in ddH2O, autoclaved
Concentration:
50 mg/ml
Particle sizes:
0.5 µm, 0.75 µm, 1.0 µm; For ordering mention the fluorescence color.
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Functional Group
Product Number
Quantity (ml)
Activation / Coupling
Product Information
Reference Number
screenMAG-Amine - Si-(CH2)3-NH2
2101-12101-5
15
Carbodiimide method Attachment: amino groups see protocol: A2/3
6.01
screenMAG-Carboxyl - Si-(CH2)3-COOH
2102-12102-5
15
Carbodiimide method Attachment: amino groups see protocol: A1
6.07
screenMAG-Cyanuric -Si-(CH2)3
2103-12103-5
15
Cyanuric chloride, auto-reactive see coupling protocol: A8
-
screenMAG-Hydrazide - Si-(CH2)3~CO-NH-NH2
2104-12104-5
15
Oxidized carbohydrats Attachment: aldehyde see protocol: A5
-
screenMAG-Hydroxyl - Si-(CH2)3-OH
2105-12105-5
15
Cyanogen bromide activation see protocol: A7
6.08
screenMAG-PEI - Si-(CH2)3-(CH2-CH2-N+H)n-CH2-CH2-NH2
2106-12106-5
15
Strong anion-exchanger
6.07, 6.08
screenMAG-Silanol - Si-OH
2107-12107-5
15
Purification of DNA/RNA, adsorption of biomolecules
6.14
screenMAG-Thiol - Si-(CH2)3-SH
2108-12108-5
15
Maleimides Attachment: thiol groups
-
screenMAG-affinity
screenMAG-affinity - bioaffine ligands are covalently bound to the magnetic-fluorescent particles. High selectivity and high capacity make these products for an ideal tool for the isolation of specific biomolecules.
Application:
Affinity chromatography
Formulation:
buffer suspension, 0.01 NaN3
Concentration:
10 mg/ml
Particle sizes:
0.5 µm, 0.75 µm, 1.0 µm; For ordering mention the fluorescence color.
screenMAG with Ligand
Product Number
Quantity (ml)
Application for:Purification / Binding
Product Information
Reference Number
screenMAG-Biotin
2201-12201-5
15
Avidin/streptavidin labeled molecules
-
screenMAG-Goat anti-mouse IgG(whole molecule)
2202-12202-5
15
Binding of secondary antibody See protocol: B4
-
screenMAG-Protein A
2203-12203-5
15
Antibodies see protocol: B2
6.10
screenMAG-Protein G
2204-12204-5
15
-
screenMAG-Streptavidin
2205-12205-5
15
Biotin-labeled molecules See protocol: B1
-