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ScreenMAG
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screenMAG screenMAG-particles are magnetic-fluorescent beads based on the well-known SiMAG technology. The advantage of both innovative techniques - the magnetic- and the fluorescence properties - provide exciting new possibilities to explore new fields of application in biosciences.   FluorescenceType FluorescenceColor Excitation Emission screenMAG/B blue 400 nm 420 nm screenMAG/G green 502 nm 525 nm screenMAG/O orange 526 nm 555 nm screenMAG/OP orange 536 nm 617 nm screenMAG/RR red 540 nm 625 nm screenMAG/R red 633 nm 672 nm Fluorescence image of screenMAG/G particles under magnetic field Fluorescence image of screenMAG/O particles under magnetic field   NOTE: The fluorescence of the screenMAG particles is only detectable on the same side where the excitation takes place. Please note that there is a difference in fluorescence observation between dissolved fluorescence molecules and solid fluorescence particles. Fluorescence spectrophotometer with a fluorescence detection unit with an angle of 90° to the excitation source will detect no or only weak fluorescence signals. screenMAG-active screenMAG-active - terminal reactive groups of the magnetic-fluorescent beads allow the convenient and efficient covalent binding of bioaffine ligands. This is a unique feature accounting for the rapidly increasing number and variation of novel and different receptor-affine biological substances. Application: Magnetic separation and fluorescence identification of targets Formulation: suspension in ddH2O, autoclaved Concentration: 50 mg/ml Particle sizes: 0.5 µm, 0.75 µm, 1.0 µm; For ordering mention the fluorescence color. top Functional Group Product Number Quantity (ml) Activation / Coupling Product Information Reference Number screenMAG-Amine - Si-(CH2)3-NH2 2101-12101-5 15 Carbodiimide method Attachment: amino groups see protocol: A2/3 6.01 screenMAG-Carboxyl - Si-(CH2)3-COOH 2102-12102-5 15 Carbodiimide method Attachment: amino groups see protocol: A1 6.07 screenMAG-Cyanuric -Si-(CH2)3 2103-12103-5 15 Cyanuric chloride, auto-reactive see coupling protocol: A8 - screenMAG-Hydrazide - Si-(CH2)3~CO-NH-NH2 2104-12104-5 15 Oxidized carbohydrats Attachment: aldehyde see protocol: A5 - screenMAG-Hydroxyl - Si-(CH2)3-OH 2105-12105-5 15 Cyanogen bromide activation see protocol: A7 6.08 screenMAG-PEI - Si-(CH2)3-(CH2-CH2-N+H)n-CH2-CH2-NH2 2106-12106-5 15 Strong anion-exchanger 6.07, 6.08 screenMAG-Silanol - Si-OH 2107-12107-5 15 Purification of DNA/RNA, adsorption of biomolecules 6.14 screenMAG-Thiol - Si-(CH2)3-SH 2108-12108-5 15 Maleimides Attachment: thiol groups - screenMAG-affinity screenMAG-affinity - bioaffine ligands are covalently bound to the magnetic-fluorescent particles. High selectivity and high capacity make these products for an ideal tool for the isolation of specific biomolecules. Application: Affinity chromatography Formulation: buffer suspension, 0.01 NaN3 Concentration: 10 mg/ml Particle sizes: 0.5 µm, 0.75 µm, 1.0 µm; For ordering mention the fluorescence color.     screenMAG with Ligand Product Number Quantity (ml) Application for:Purification / Binding Product Information Reference Number screenMAG-Biotin 2201-12201-5 15 Avidin/streptavidin labeled molecules - screenMAG-Goat anti-mouse IgG(whole molecule) 2202-12202-5 15 Binding of secondary antibody See protocol: B4 - screenMAG-Protein A 2203-12203-5 15 Antibodies see protocol: B2 6.10 screenMAG-Protein G 2204-12204-5 15 - screenMAG-Streptavidin 2205-12205-5 15 Biotin-labeled molecules See protocol: B1 -

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