SiMAG SiMAG particles are magnetic silica beads with superparamagnetic or ferromagnetic properties and possess either a highly porous or a non-porous silica surface. The beads are available with diameters of 0.5 µm to 1 µm and are easily separable by magnetic batch procedures. The surface area provides a high binding capacity of biomolecules such as purified DNA/RNA or of bioaffine ligands, e.g. antibodies or proteins. SiMAG beads have an inert and stable solid-phase silica matrix without autofluorescence and are autoclaved for sterile applications. SiMAG beads can be used for ion-exchange, hydrophobic or covalent binding of biological substances due to the chemical modification of the silica surface with a variety of functional groups. The hydrophilic character, the mechanical stability, the resistance to microbiological degradation, and excellent reproducibility are SiMAG's major advantages in biotechnology applications.   SiMAG - physico chemical characteristics Composition: Aqueous dispersion of magnetic silica particles Core: Maghemite Size: 0.5 µm, 0.75 µm, 1 µm Matrix: Silica (non porous), (highly porous on request) Surface Area: ~ 50 m2/g Number of Particles: 1.8 x 1012/g (for size: 1 µm) Density: 2,25 g/cm3 Type of magnetization Superparamagnetic, (ferromagnetic on request)                     SiMAG - basic SiMAG-Silanol beads have an unmodified silica surface with terminal negatively charged silanol groups. These superparamagnetic beads are applicable for a wide range of applications in biochromatography, e.g. purification of DNA/RNA, adsorption of biomolecules or isolation of basic proteins. chemicell offers three different kinds of SiMAG-Silanol particles: SiMAG/F-Silanol, SiMAG/T-Silanol and SiMAG/N-Silanol caused by different silica coating procedures which generate different silica layers with a diverse distribution and formation of silanol groups (e.g. silanol, di-silanol, tri-silanol groups). As a result of this these different kinds of SiMAG-Silanol particles will offer a different surface charge and charge distribution which will influence the binding property to DNA, RNA and proteins. Please contact and inform us for which application and which target molecule you will use the SiMAG-Silanol beads.   Application: Purification of DNA/RNA; adsorption of biomolecules; isolation of basic proteins     Formulation: suspension in ddH2O, autoclaved Concentration: 50 mg/ml Particle sizes: 0.5 µm, 0.75 µm, 1.0 µm Functional Group        Product         Number    Quantity (ml)    Product Information   Reference Number SiMAG-Silanol - Si-OH 1101-11101-5 15 1.12, 1.30,1.55, 1.66,3.01, 4.08,7.01, 7.02                                    SiMAG-DNA   SiMAG/K-DNA and SiMAG/MP-DNA beads have been developed for automatic DNA/RNA separation such as genomic DNA, plasmid DNA, total RNA and PCR products. DNA/RNA bind to these porous silica surface under high salts conditions (5 M guanidinium thiocyanate). The superparamagnetic SiMAG/K-DNA beads and the ferromagnetic SiMAG/MP-DNA beads have excellent magnetic properties and are therefore most suited for automatic DNA/RNA separation / purification. Since different robot systems work with different processing methods or magnetic separation systems, either superparamagnetic or ferromagnetic SiMAG-DNA beads will lead to optimal results. Please contact us for technical support: E-Mail: info@chemicell.com   Application: For automatic purification of DNA/RNA Formulation: suspension in ddH2O, 20% ethanol Concentration: *100 mg/ml / **200 mg/ml Particle sizes: *1.0 µm / **2.0 µm Functional Group Product        Number        Quantity (ml)   Product     Information        Reference Number *SiMAG/K-DNAsuperparamagnetic - Si-OH 1103-11103-5 15   1.07 **SiMAG/MP-DNAferromagnetic - Si-OH 1102-11102-5 15   1.07, 1.53            SiMAG-DNA – high porous silica surface                                           SiMAG - ionex   SiMAG-ionex - terminal ion-exchange groups are reversibly to charged groups of biomolecules and can be eluted with salt- or pH-gradient. Ion-exchange separation can be used for many applications such as the isolation of proteins, antibodies, DNA/RNA and other biomolecules. Anion-exchange magnetic particles bind selectively to the negatively charged membrane groups of cells, bacteria or viruses which can be separated by magnetic forces. Application: Separation of antibodies, proteins or peptides Formulation: suspension in ddH2O, autoclaved Concentration: 50 mg/ml Particle sizes: 0.5 µm, 0.75 µm, 1.0 µm   Ion-Exchanger Type     Product Number           Quantity (ml)         Product Information        Reference Number SiMAG-Carboxyl weak cation-exchanger - Si-(CH2)3-COOH 1201-11201-5 15   1.02, 1.10, 1.16,1.21, 1.23, 1.24,1.31, 1.34, 1.47,1.54, 1.57, 3.02,5.03, 5.05, 5.06,5.12 SiMAG-Sulfon strong cation-exchanger - Si-(CH2)3-SO3H 1202-11202-5 15   1.08 SiMAG-Phosphonate strong cation-exchanger - Si-(CH2)3-PO3H2 1205-11205-5 15   - SiMAG-DEAE weak anion-exchanger - Si-(CH2)3-CH2-CH2-N+H(CH2-CH2)2 1203-11203-5 15   1.05, 1.08, 1.41,1.67, 2.07 SiMAG-PEI strong anion-exchanger - Si-(CH2)3-(CH2-CH2-N+H)n-CH2-CH2-NH2 1204-11204-5 15   1.05, 1.06, 1.43,1.67, 2.07 SiMAG-Q strong anion-exchanger - Si-R-CH2-CHOH-CH2-N+(CH3)3 1206-11206-5 15   1.08, 2.07 SiMAG-PVPY weak anion-exchangePoly(4-vinylpyridine) 1207-11207-5 15   -   SiMAG - hydrophobe   SiMAG-hydrophobe - is a useful tool for the magnetic separation of biomolecules based on differences in surface hydrophobicity. The hydrophobe regions from proteins, peptides, enzymes or nucleic acids are preferentially targeted by hydrophobic interaction. The binding strength depends on the ionic conditions, the detergents, the pH and the temperature. Application: Separation of proteins, peptides and enzymes Formulation: suspension in ddH2O, autoclaved Concentration: 50 mg/ml Particle sizes: 1.0 µm   Functional Group Product        Number             Quantity (ml)   Product  Information Reference Number SiMAG-Octadecyl (C18) - Si-(CH2)17CH3 1301-11301-5 15   2.01, 2.03 SiMAG-Octyl (C8) - Si-(CH2)7CH3 1302-11302-5 15   2.01, 2.02, 2.03,2.04, 2.05 SiMAG-Propyl (C3) - Si-CH2CH2CH3 1303-11303-5 15   2.01, 2.03 SiMAG-Ethyl (C2) - Si-CH2CH3 1304-11304-5 15   2.01 SiMAG-Methyl (C1) - Si-CH3 1305-11305-5 15   2.01   SiMAG - active   SiMAG-active - terminal reactive groups allow the convenient and efficient covalent binding of bioaffine ligands. This is a unique feature accounting for the rapidly increasing number and variation of novel and different receptor-affine biological substances.See: Coupling-Protocols Application: Activated particles for custom-made covalent binding of biological substances Formulation: suspension in ddH2O, autoclaved Concentration: 50 mg/ml Particle sizes: 0.5 µm, 0.75 µm, 1.0 µm Functional Group Product Number Quantity (ml)   Activation / Coupling Product Information Reference Number SiMAG-Amine - Si-(CH2)3-NH2 1401-11401-5 15   Carbodiimide method Mannich reaction see coupling protocol: A2/3 1.02, 1.16, 1.30,1.32, 1.47, 5.06 SiMAG-Carboxyl - Si-(CH2)3-COOH 1402-11402-5 15   Carbodiimide method see coupling protocol: A1 1.02, 1.10, 1.16,1.21, 1.23, 1.24,1.31, 1.34, 1.47,1.54, 1.57, 3.02,5.03, 5.05, 5.06,5.12 SiMAG-Cyanuric -Si-(CH2)3 1403-11403-5 15   Cyanuric chloride, auto-reactive see coupling protocol: A8 1.47, 2.10 SiMAG-Hydrazide - Si-(CH2)3~CO-NH-NH2 1404-11404-5 15   Oxidized carbohydrats see coupling protocol: A5 1.23, 1.47, 1.49,2.06, 2.09 SiMAG-Hydroxyl - Si-(CH2)3-OH 1405-11405-5 15   Cyanogen bromide activation see coupling protocol: A7 1.42, 1.47 SiMAG-PGL - Si-(CH2)3 1406-11406-5 15   For covalent coupling of bioligands, see coupling protocol: A4 1.47, 3.12 SiMAG-Thiol - Si-(CH2)3-SH 1407-11407-5 15   Maleimides 1.18, 1.47 SiMAG-Bromoacetyl -Si-O-R-CO-CH2-Br 1408-11408-5 15   For binding of SH-groups containing bioligands see coupling protocol: A14 - SiMAG-Chitosan -Si-O- 1409-11409-5 15   For separation of peptides and enzymes, selective binding of metal ions -   SiMAG - affinity   SiMAG-affinity - bioaffine ligands are covalently bound to magnetic particles. High selectivity and high capacity make these products for an ideal tool for the isolation of specific biomolecules. Application: Affinity chromatography Formulation: buffer suspension, 0.01 NaN3 Concentration: 10 mg/ml Particle sizes: 1.0 µm   SiMAG with Ligand   Product Number   Quantity (ml)     For Purification   Product Information        Reference Number SiMAG-Albumin 1501-11501-5 15   Bilirubin, amino acid - SiMAG-Benzamidine 1502-11502-5 15   Thrombin, serine proteases - SiMAG-Biotin 1503-11503-5 15   Avidin/streptavidin labeled molecules - SiMAG-Boronic Acid 1504-11504-5 15   Ribonucleotides, sugars via cis-diols See protocol: B14 1.28, 1.45, 1.46 SiMAG-α-Casein 1505-11505-5 15   RNA polymerase - SiMAG-Concanavalin A 1506-11506-5 15   Bacteria, glycoproteins 1.36 SiMAG-DNA(calf tymus DNA) 1507-11507-5 15   mRNA, rRNA - SiMAG-Gelatin 1508-11508-5 15   Fibronectin - SiMAG-Glutathione 1509-11509-5 15   GST fusion proteins See protocol: B6 - SiMAG-Goat anti-mouse IgG(whole molecule) 1510-11510-5 15   For binding of secondary antibody See protocol: B4 - SiMAG-Goat anti-rabbit IgG(whole molecule) 1520-11520-5 15   For binding of secondary antibody See protocol: B11 1.64 SiMAG-Heparin 1511-11511-5 15   Lipoprotein, lipase, RNA-polymerase 1.38 SiMAG-IDA/Nickel 1512-11512-5 15   For Immobilized Metal ion Affinity Chromatography (IMAC) See protocol: B5 1.04, 1.09, 1.44 SiMAG-Oligo (dT)n 1513-11513-5 15   mRNA See protocol: B3 - SiMAG-Polymyxin 1514-11514-5 15   Removal of endotoxines 1.11, 1.26 SiMAG-Protein A 1515-11515-5 15   Antibodies See protocol: B2 1.45, 1.46, 1.58,1.60 SiMAG-Protein G 1516-11516-5 15   - SiMAG-Streptavidin 1517-11517-5 15   Biotin labeled molecules See protocol: B1 1.42, 1.56, 1.64,3.08 SiMAG-Trypsin 1518-11518-5 15   Protein- and Fab fragments of IgM - SiMAG-Trypsin Inhibitor 1519-11519-5 15   Trypsin -