Description Magnetofection™ is a novel, simple and highly efficient method to transfect cells in culture. It exploits magnetic force exerted upon gene vectors associated with magnetic particles to draw the vectors towards, possibly even into, the target cells. In this manner, the full vector dose applied gets concentrated on the cells within a few minutes so that 100% of the cells get in contact with a significant vector dose. This has several important consequences: Greatly improved transfection rates in terms of percentage of cells transfected compared to standard transfection. Up to several thousand fold increased levels of transgene expression compared to standard transfections upon short-term incubation. High transfection rates and transgene expression levels are achievable with extremely low vector doses, which allows to save expensive transfection reagents. Extremely short process time. A few minutes of incubation of cells with gene vectors are sufficient to generate high transfection efficiency, compared to several hours with standard procedures.               Technology Magnetofection™ Reagents As the manufacturer of the Magnetofection™ technology, chemicell offers two types of ready-to-use Magnetofection™ reagents. PolyMAG is a universally applicable magnetic particle preparation for high efficiency nucleic acid delivery. It is mixed in a one-step procedure with the nucleic acid to be transfected and has been used successfully with plasmid DNA, antisense oligonucleotides and siRNAs. CombiMAG is a magnetic particle preparation designed to be combined with any commercially available transfection reagent such as polycations and lipids and can be associated with plasmid DNA, antisense oligonucleotides, siRNAs or viruses. It allows you to create your own magnetic gene vector based on your favourite transfection reagent. Purchaser Notification The Magnetofection™ reagents and all of its components are developed, designed, intended and sold for research use only. They are not to be used for human diagnostic or any drug intended. Magnetofection™ are registered trademark top Applications Magnetofection™ is generally applicable for adherent cells and has been tested with a variety of immortalized cell lines and primary cells listed below. If a particular cell type or cell line is not listed this does not mean that Magnetofection™ would not work. Also for the cells listed, some reagents have not been tested so far, as indicated by "n.d." (not determined). The CombiMAG reagent can be combined with any polycationic and lipidic transfection reagent, and also with adenoviral and retroviral vectors. In some cases, references are made in the footnotes to very successful combinations with commercially available reagents that have been tested so far.     Type of nucleic acid / virus PolyMAG CombiMAG Plasmid DNA Antisense Oligonucleotides siRNA Adenovirus n.d. Retrovirus n.d.                                 Effectene (Qiagen)                                    Effectene + CombiMAG   Primary human umbilical vein endothelial cells positioned on the MagnetoFACTOR plate were incubated for 15 min with a Cy3 fluorescencelabeled antisense-oligonucleotide complexed with Effectene™ (Qiagen; left) or Effectene™ + CombiMAG (right).  (Data kindly provided by F. Kroetz. Ludwig-Maximilians University Munich)  Adherent cells Cell Line Cell Type Source PolyMAG CombiMAG* Reference Number HeLa cervix carcinoma human + + 9.030, 9.024, 9.072, 9.012, 9.111, 9.003, 9.005, 9.016 HEK 293 kidney human + + 9.013, 9.064, 9.054 BHK kidney hamster n.d. + - CHO ovarian hamster + + 9.036, 9.002, 9.005, 9.006 NIH 3T3 fibroblasts mouse + + 9.003, 9.072, 9.091, 9.002, 9.005, 9.004 16HB140 airway epithelium human + n.d. 9.010 EJ28 bladder cancer human + n.d. - HBL-100 breast human n.d. + - MCF7 breast adenocarcinoma human + + 9.014, 9.040, 9.041, 9.115 MCS7 breast adenocarcinoma human + n.d. - HCT 116 colon adenocarcinoma human n.d. + - U373 astrocytoma human + n.d. 9.056 U87 glioblastoma human + n.d. 9.056 U251 glioblastoma human + n.d. 9.056 T98G glioblastoma human + n.d. 9.056 YK6-1 glioblastoma human + n.d. 9.056 YH-13 glioblastoma human + n.d. 9.056 A549 alveolar basal epithelial human + + 9.075, 9.101 A172 glioblastoma human + n.d. 9.056, 9.074 LN229 glioblastoma human + + 9.074 LN18 glioblastoma human + + 9.074 SW480 colon adenocarcinoma human n.d. + - LoVo colon adenocarcinoma human n.d. + - HCT15 colon adenocarcinoma human n.d. + - CEMx174 hybrid T/B-lymphocytes human n.d. + 9.060 CHO ovarian human + + - A431 epidermoid carcinoma human n.d. + - HT-1080 fibrosarcoma human + n.d. - HFF foreskin fibroblast human + + - Hep G2 hepatocellular carcinoma human + + 9.012, 9.048 HaCat keratinocytes human n.d. + - 293 T kidney human + + 9.030 293 T-17 kidney human n.d. + 9.030 SK-MES-1 lung carcinoma human + n.d. - MRC 5 lung, embryonic human + + - MeWo melanoma human n.d. + - NYGM cerebral glioblastoma human n.d. + 9.056 SK-MEL-28 melanoma human n.d. + - MEP mammalian epithelial human n.d. + 9.047 MOLT-4 acute lymphoblastic leukemia human n.d. + 9.044 SHSY-5Y neuroblastoma human n.d. + 9.111, 9.050 SV40 transformed fibroblasts human + n.d. 9.027 A549 Non-small cell lung carcinoma human n.d. + 9.057, 9.101 SaOS-2 osteo sarcoma human n.d. + - BTK-143 osteo sarcoma human n.d. + - BEAS-2B lung epithelial cells human + n.d. 9.012 181RDB pancreatic human n.d. + - HN12 Head, neck carcinoma human n.d. + 9.028 PC-3 prostate carcinoma human n.d. + - H9 lymphoblastoid human n.d. + 9.077 H295R adrenocortical carcinoma human n.d. + 9.058, 9.059, 9.037 HOS osteosarcoma human n.d. + 9.077 HSG salivary gland, submandibular human n.d. + - He99 lung cancer human + + 9.100 HMEC-1 microvascular endothelial human + n.d. 9.038, 9.092 NCI-H441 lung adenocarcinoma epithelial human + + 9.073 NCI-H292 mucoepidermoid carcinoma human + n.d. 9.065, 9.095, 9.112 NCI-H82 small cell lung cancer human + n.d. - SK-N-BE2 neuroblastoma human + n.d. 9.031 MDAMB231 breast adenocarcinoma human + n.d. 9.035 ECV-304 urinary bladder carcinoma human n.d. + - 11.5dpcXY gonad explants mouse n.d. + 9.109 Cal27 oral adenosquamous carcinoma mouse n.d. + 9.028 CT-26 colon carcinoma mouse + n.d. 9.003, 9.005 C127 murine mammary tumor mouse n.d. + 9.042 F9 embryonal carcinoma mouse + n.d. - L929 fibroblast, connective tissue mouse + n.d. 9.016 MEF fibroblast mouse + n.d. 9.039, 9.098 HT-22 hippocampal neuroblast mouse n.d. + - mIEnd Mesenteric lymph node mouse n.d. + 9.085 mIC-(cl2) intestine mouse n.d. + - B16F10 melanoma mouse + n.d. 9.076, 9.005 N2a neuroblastoma mouse + n.d. 9.063, 9.086 NS20Y neuroblastoma mouse n.d. + - bTC-tet B-cell line mouse + + 9.020 Hepa1c1c7 murine hepatoma mouse + n.d. 9.084 SVEC murine cell line mouse n.d. + 9.028 OP9 bone marrow-derived stromal mouse n.d. + 9.047 SM 10 trophoblast mouse n.d. + - STC-1 enteroendocrine I cells mouse + + 9.033 A7r5 aortic smooth muscle rat n.d. + 9.094 PC12 adrenal pheochromocytoma rat + n.d. - PC6-3 daughter cell line of PC12 rat + n.d. - C6 glioma rat n.d. + - H4IIE hepatoma rat n.d. + 9.062 L6 skeletal muscle cell rat + n.d. - RIE1-α5 intestinal rat + n.d. 9.088 HNSCC Head, neck carcinoma rat n.d. + 9.015, 9.028, 9.051 RV vein rat + n.d. - COS-7 kidney monkey + + 9.030 CV-1 kidney monkey + n.d. - COS-1 kidney monkey n.d. + - Vero kidney monkey n.d. + 9.045, 9.046, 9.016 B11 (UV) resistant ovary hamster n.d. + 9.028 V79 lung fibroblasts hamster + n.d. - PT-11 kidney bovine n.d. + - MDCK kidney canine + n.d. 9.067 CRFK kidney cat n.d. + - VSa13 bone (chondrocyte-like) fish + n.d. - AM-C6SC8 kidney pig n.d. + - - fibroblasts xenopus + n.d. - EMC mesenthelial. Cells (epicard)   n.d. + -   Suspension cells Cell Line Cell Type Source PolyMAG CombiMAG* Reference number THP-1 acute myeloid leukemia human n.d. + - Jurkat acute T-cell lymphoma human n.d. + 9.044, 9.073 BL-41 B-cell lymphoma human + n.d. - K562 chronic myeloid lymphoma human n.d. + 9.002 P815 mastocytoma mouse n.d. + - U937 histiocytic lymphoma human + + - Primary cells Cell Type Source PolyMAG CombiMAG* Reference Number endothelial cells (cord blood) human + n.d. 9.054 aortic endothelial cells human + n.d. 9.028, 9.032 epithelial (HUVEC) human + n.d. 9.029, 9.034, 9.092, 9.113, 9.114, 9.003, 9.007, 9.008, 9.021, 9.029, 9.034, 9.005, 9.004 fibroblasts human + + - fibroblasts, diploid human + + - gastric gland human + + - adherent gastric cells human n.d. + 9.069 gastric myofibroblasts human + n.d. 9.043 glioma human + n.d. - keratinocytes human n.d. + 9.005 LNCaP human n.d. + - lymphocytes human + + 9.005, 9.102 mammal epithelium human n.d. + - nasal airway epithelium human + + 9.005 pancreatic tumor human + n.d. - peripheral blood lymphocytes human + + 9.005, 9.102 stroma cells (endometrium) human n.d. + - T-cells human + n.d. 9.102, 9.089 chondrocytes human + + 9.107, 9.022, 9.024 trophoblastic cells human n.d. + - fibroblasts (MEF) mouse n.d. + 9.018, 9.053 neurons mouse + + 9.071 vagal afferent neurons mouse n.d. + 9.071 adherent gastric cells mouse n.d. + 9.061 peripheral blood lymphocytes mouse n.d. + - myoblasts mouse n.d. + 9.053 T-cells mouse + n.d. - hippocampal neurons rat + + 9.017, 9.070, 9.050, 9.052, 9.093, 9.090, 9.105, 9.104 aortic smooth muscle cells rat n.d. + 9.094 cardiomyocyte rat n.d. + 9.055 hepatocytes rat n.d. + - epithelial (HUVEC) rat + + 9.028, 9.054 cortical neurons rat n.d. + 9.052, 9.063, 9.097 aortic endothelial cells bovine + n.d. - carotid artery smooth muscle bovine + n.d. - chromaffine cells bovine n.d. + - lens bovine + n.d. - airway epithelium pig n.d. + - chondrocytes pig + + - fibrochondrocytes pig + + - pSM, smooth muscle pig n.d. + -   * Corresponds to successfully transfected by CombiMAG in association with different commercial transfection reagents such as FuGENE™ (Roche), Lipofectamine™, METAFECTENE™ (Biontex GmbH) and DMRIE-C™ (Invitrogen); adenovirus; Effectene™ (Qiagen) n.d. = not determined  Available Kits  Storage: All components of the Magnetofection™ kit should be stored at room temperatur (20-25°C). After first use store the kit at +4°C. Do not freeze the magnetic nanoparticles Do not add anything to the stock solution of magnetic nanoparticles Shipping Conditions: Room Temperature    MagnetoFACTOR-96 plate MagnetoFACTOR-24 plate                               The MagnetoFACTOR-96 plate its special geometry not only produces strong magnetic fields under each well of 96-well plates but is also applicable for other plate formats T-75 culture flasks, 6- and 12-well plates. In the larger plate formats, the MagnetoFACTOR plate will produce a pattern of higher and lower densities of transfected cells according to the geometry of the magnetic field lines. The MagnetoFACTOR-24 plate is special designed for the 24-well format.   The MagnetoFACTOR plates are compatible for well plates of the most manufacturer.   SPECIAL OFFER As an introductory offer you will receive one vial each of PolyMAG and CombiMAG sufficient for 200 µg of DNA each for free in combination with the purchase of the MagnetoFACTOR plate. This will enable you to optimize your Magnetofection™ in up to 3000 transfection experiments (96-well format).