본문 바로가기 주메뉴 바로가기
홈
Distribution Product/Sercive

공공기관

Mobility Shift Assay(MSA)/IMAP™ Kinase Assay Services
Mobility Shift Assays/IMAP™ assays directly monitor phosphorylation by measuring the non-phosphorylated and phosphorylated substrate in the kinase reaction. The potency and selectivity of your compounds are assessed against target kinases you select. All kinases utilized in our assays are produced and guaranteed to be of highest quality, enabling us to deliver high quality data to you. The ATP concentration in our assays is set around the Km value. For enhanced understanding of selectivity and allosterism, many assays have also been validated using 1mM ATP. For slow binding compounds, we offer assays pre-incubating the kinase with your compounds.
Features include:
  • Both IC50 determinations and the percent inhibition ratio of your compounds
  • ATP concentration approximating Km are our routine service assays
  • Many assays are also available at 1 mM ATP
  • Carna’s preincubation service is utilized to study slow binding compound
ONLINE INQUIRY +
Details Link +

Detail

Target별로 선택 가능


- 모든 kinase tagrets

- Tyrosine kinase targets

- Serine/Threonine kinase targets

- Wild type kinase targets

- Cascade assay targets



ATP 농도 = Km 값 부근, 1 mM 측정 선택 가능


1 mM ATP 농도를 이용하여 보다 생체에 가까운 환경에서 측정함으로써, 화합물의 특성 식별 가능


 


Slow binding compound에 최적인 pre-incubation 서비스 


target kinase로의 결합 속도가 매우 느린 화합물의 활성 평가에 최적 (실온에서 30분 동안 pre-incubation을 진행하기 때문에, 실온에서도 활성의 안정성이 확인된 kinase로만 실시)


 

p38a kinase 저해제 작용의 경시적 변화 (ATP =  1 mM)


p38a kinase에 대한 화합물의 저해 효과를 시간별로 측정. 일반적인 저해제인 SB202190은 반응 시작 직후부터 kinase 반응의 저해를 나타낸 것에 비해, slow binder로 알려진 BIRB796은 시간 경과에 따라 저해율이 점차 상승함을 보여줌.



Order Information

Category Product Name Cat No.
등록된 게시물이 없습니다.

Related Products

Search