prepGEM Universal Kit
Extracts DNA from blood, saliva, and tissue including animal (ear punches, mouse tails, insect) and cell culture.
Produces single-stranded DNA for STR, PCR, qPCR, whole genome amplification, amplicon sequencing.
Perfect for CRISPR/Cas9 gene editing with NGS.
Each extraction kit contains prepGEM, Histosolv, BLUE buffer, RED+ buffer, and ORANGE+ buffer.
prepGEM Universal is MicroGEM’s comprehensive solution for effortless high-yield DNA extraction from blood, saliva, and tissues.
It is also perfectly suited for extracting DNA from cell cultures.
All within a single tube, this method ensures no loss of DNA, and as a result, it excels with low cell number extractions.
This kit is an excellent tool for researchers performing confirmatory genotyping of transfected cells following CRISPR/Cas9 gene editing.
prepGEM Universal utilizes our thermophilic proteinase by activation with a simple temperature change. The whole process is easily automated in a single tube using standard laboratory equipment.
A standard thermocycler can be programmed to carry out the extraction.
Features include:
- Stream-lined approach provides DNA in approximately 15 minutes
- Inhibitor-free solutions mean no harsh chemical washes or multiple steps are required
- Closed-tube extraction protects the integrity of the sample
- Simplified, hands-off workflow frees up technician time, reduces lab costs, and significantly reduces hazardous/plastic waste