PDQeX forensicGEM Sperm Kit
Generates closed-tube, hands-free purified double-stranded DNA
Ideal for high throughput screening and STR, PCR, and qPCR
forensicGEM Sperm is our new name for our previous forensicGEM Sexcrime kit – same product, same formulation – just a new name.
Each kit contains PDQeX forensicGEM, Acrosolv, ORANGE+ buffer, and tubes.
The PDQeX forensicGEM Sperm kit offers a simple hands-off method for DNA extraction and purification of sperm.
The purified DNA extraction is proven for Y chromosome-specific real time PCR assay, and for sperm lysis as part of a differential extraction procedure.
Using MicroGEM’s PDQeX Nucleic Acid Extractor, there are only two simple steps – quick sample prep and temperature-controlled DNA extraction and purification.
This closed-tube, automated approach does not use inhibiting chemicals, such as SDS, mercaptoethanol and DTT, so no additional wash steps are required.
DNA yields are preserved and processing time is significantly reduced. This approached generates purified double-stranded DNA ready for high-throughput screening and is ideal for STR, PCR and qPCR applications.
Features include:
- Comprehensive lysis of sperm cells in 20 minutes
- Simplified, hands-off workflow with no additional purification steps
- Extracts DNA from sperm in very small volumes and with very few sperm
- Reduced handling protects the integrity of the sample