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In Vivo & In Vitro Electroporator
CUY21EDIT II is the true succesor of the best electroporator CUY21EDIT, which has a lot of great performance.

CUY21EDIT II is easy to be used and generates the accurate pulses.

As well as succeeding the great function of CUY21EDIT, BEX technology enable CUY21EDIT II to generate every pattern of the pulses for both of in vivo and in vitro. Moreover, CUY21EDIT II is the first electroporator to generate constant current pulses.

CUY21EDIT II performs combination of constant voltage pulses and constant current voltage pulses, which leads to a whole new electroporation world !
Features include:
  • The genuine successor for CUY21EDIT, which is the best in vivo electroporator
  • CUY21EDIT II generates the accurate squere pulse for in vivo and decaying pulse for in vitro
  • CUY21EDIT II is the true electroporator, which two power units suitable for in vivo and in vitro respectively
  • CUY21EDIT II is the first electroporator to generates constant current pulses.
  • Constant current pulses is the best for GONAD as well as in vivo electroporation.
  • CUY21EDIT II is half as heavy as CUY21EDIT
  • Easy to use with 5.7 inch touch panel.
  • All data of executed voltage and current of generated pulses are recorded.
  • The pulse records is exported to a USB memory
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Detail

● 모든 패턴의 pulses 


- CUY21EDIT II는 CUY21EDIT에서 가능한 Square pulses 뿐만 아니라 decaying pulses도 가능하고, CUY21EX에서 가능한 dual pulses도 가능

즉,

1. Poration Pulses (Pp): 세포 표면에 매우 작은 holes을 만들어 electroporation

2. Driving Pulses (Pd): 세포 안에 DNA, RNA 같은 물질을 옮김 


PpOnly - CUY21EDIT II can change the polarity of the squre pulses alternately.


PpPd(+-) - CUY21EDIT II generates dual pulses with square pulses and can change the decay rate of the voltage.


PpPd(+-)Capacitor - CUY21EDIT II can change the polarity of the decaying Driving Pulses in Dual pulse mode alternately. The convert of the capacitance of the condenser enable decay rate to be changed.


ppPd(alt) - CUY21EDIT II can also change the polarity of the square Driving Pulses in Dual pulse mode alternately. The decay rate can be changed. 


● 사용하기 쉬운 5.7-inch touch panel 



▶ Applications ◀ 


♣ GONAD (Genome editing via Oviductal Nucleic Acids Delivery)


3단계로 완성~



Tweezer-like electrode

 

constantcurrent1   constantcurrent2 


§ 결과 


2-Cell embryos after electroporation


 






♣ Zygote Electroporation (GEEP method) 


High throughput and efficient CRISPR/Cas9-based mouse genome editing by RNA electroporation to zygote.


genome_editing_result 


Fgf10 gene knockout mouse by Cas9 mRNA/gRNA electroporation

Fgf10 monozygous mutant embryo have an easily detectable limbless phenotype. Electroporation of Cas9 mRNA (Cas9 Protein) and gRNA enables genome editing of Fgf10.






♣ in vitro Electroporation (culture cell electroporation) 


 Human Tissue-Derived Cells

 Name of Cell

 Tissue

 Viability (%)

 Efficiency (%)

 MG-63

 Bone

 70

 87.5

 KG-1

 Bone marrow

 75

 70

 THP-1

 Peripheral blood

 50

 60

 Kasumi-1

 Blood

 45

 65

 RAJI

 Burkitt lymphoma

 60

 75

 MOLT-4

 Peripheral blood

 82.5

 95

 LoVo

 Colon

 60

 62.5

 HT-1080

 Sarcoma

 80

 95

 Ramos(RA1)

 Burkitt lymphoma

 70

 70

 CACO-2

 Colon

 70

 50

 HCT116

 Colon

 85

 90

 Saos-2

 Bone

 90

 87.2

 HaCaT

 Skin

 80

 75

 NCI-H1299

 Lung

 85

 95

 U20S

 Bone

 80

 87

 A-172

 Brain

 85

 90

 HCT-15=DLD-1 Colon 70 80
 NUGC-3 Stomach 75 75
 PC-3 Prostate, Bone meta 75 92
 U937 Pleural effusion 55 60
 MDA-MB-231 Breast; Mammary gland 70 75
 SK-BR-3 Breast; Mammary gland 80 70
 HEK293 Kidney 76 100

 293T

 Kidney

 100

 94

 A549 Lung 87.5 60
 HeLa Cervix 71 58
 HL60 Peripheral blood 80 90
 HuH-7 Liver N/C 100
 Jurkat Blood 44 85
 MCF-7 Pleural effusion 78.8 64
 NALM-6 Blood 34.1 56

 PANC-1

 Pancreas; Duct

 96

 69

 HUV-EC-C Umbilical cord, Vein 42.5 82.4
 RPMI8226 Peripheral blood 40 67.5
 Daudi Burkitt lymphoma 70 50
 TIG-1 Fetus lung 75 80
 A-431 Epidermis 75 63
 LNCaP Prostate 42 100
 LK-2 Lung 70 71

 K562

 Pleural effusion

 75

 100

 HSC-3 Tongue 86.3 7.9
 A2058 Melanoma 85 75
 HepG2 Liver 60 60
 MRC-5 Fetus lung 65 75
 T24 Bladder 67.5 85
 Ca Ski Uterine cervix 75 55
 Mewo Skin 70 80
 A375 Skin 70 80
 PC9 Lung 60 70
 SH-SY5Y Bone marrow 60 65
 Mouse Tissue-Derived Cells
 Name of Cell Tissue  Viability (%)  Efficiency (%) 
 P-815 Tumor 50 70
 B16-F10 Skin 55 60
 LLC Lung 70 80
 MC3T3-E1 Newborn, Calvaria 75 80
 Hepa 1-6 Liver 75 85
 RAW264.7 Ascites 45 45
 Colon-26 Rectum 80 80
 NMuMG Breast, Mammary gland 60 50
 MIN6b Pancreas 87.5 87.5
 Neuro-2a Brain 46 93
 L Adipose, Areolar 46 93
 3T3-L1 Embryo 51 82
 NIH3T3 Embryo 64 100
 Colon 26 subline Colon 93.4 91
 C2C12 Muscle 80 75
 Rat Tissue-Derived Cells
 Name of Cell Tissue  Viability (%)  Efficiency (%)
 C6 Brain - 95
 Chinese Hamster Tissue-Derived Cells
 Name of Cell Tissue Viability (%) Efficiency (%)
 CHO-K1 Ovary 83 100
 Others
 Name of Cell Tissue Viability (%) Efficiency (%)
 COS-1 Kidney 100 100
 COS-7 Kidney 69.1 100
 MDCK(NBL-2) Kidney 90 85




 

Order Information

Category Product Name Cat No.
BEX   In vivo and in vitro electroporator CUY21EDIT II
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