PromethION
PromethION offers the same real-time, long-read, direct DNA and RNA sequencing technology as MinION and GridION, at much larger scale.
You may find PromethION suitable for large scale projects such as population-scale sequencing or plant genomics, The PromethION 24 and PromethION 48 systems offer the ability to run 24 and 48 flow cells respectively.
The system allows on-demand sequencing, using any flow cell at any time.
Users can start and stop running individual experiments as required, The PromethION hardware consists of 2 versions, the PromethION 24 (P24) can operate up to 24 flow cells at once and the PromethION 48 (P48) can operate up to 48 flow cells at once.
Features include:
- Each PromethION flow cell allows up to 3,000 nanopores to be sequencing simultaneously
- Using current chemistry (early 2019), the best internal yield of a single flow cell is ~220 Gb and the best external result 148 Gb. Yield will depend on the application, extraction and preparation methods
- PromethION uses the same nanopore sequencing technology as MinION/GridION:
- > PromethION flow cells are compatible with PromethION 24 and PromethION 48 (and also PromethION Beta)
- > Flow Cells can be used with gDNA, RNA and cDNA Library preparation kits
- > Sample input amount is the same as MinION/ GridION
- > Analysis and informatics same as other Nanopore data