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Laminin Mouse   This highly purified preparation of mouse Laminin I increases cell adhesion, migration, growth, and differentiation. It is composed of 111 chains with a total MW of 800 kD and is used for the coating of culture dishes.Source: Murine Engelbreth-Holm-Swarm (EHS) tumor Storage Buffer: Dulbecco‘s Modified Eagle‘s Medium with 10 μg/ml gentamycin sulfate Storage: Store at -20° C or at -80° C in a manual defrost freezer Purity: Purity > 90 % by SDS-PAGE   Description Size Product number Laminin from mouse 1 mg P06-20501   Specifications Functional assays Supports the formation of neuronal filaments of NG108-15 cells in a neurite outgrowth assay Sterility testing No bacterial or fungal growth detected after incubation at 37° C for 14 days following USP XXIV, Chapter 71 sterility testing No mycoplasma contamination detected by PCR Endotoxin concentrations < 20 EU/ml by LAL assay Coating procedure The recommended working concentration is 0.05 - 10 µg/cm² of growth surface (0.05 - 10 µg/ml) depending on cell type. a. Thaw stock solution on ice for several hours. Place plates on ice and pre-chill pipette tips.Distribute the solution to completely cover the bottom of the wells.b. The following table gives suggested volume required per well:   Plate Type Volume Laminin per Well 6 wells (or 35 mm dish) 1 ml 24 wells 200 µl 48 wells 50 µl 48 wells 20 µl   c) Incubate the plates at 37° C for 1 hour. In the laminar flow hood, remove excess liquid from the wells of the tissue culture plate. Rinse the wells once with tissue culture medium and then add your cells.

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