Endopan 3   Endopan 3 ready-to-use is a specially developed medium for the in vitro culture of human endothelial cells containing all components necessary for optimal growth. It is designed for use in an incubator at 37° C with a 5% CO2 atmosphere.Endopan 3 kit is provided with FBS and supplements in separate sterile packing. This will enable the user to prepare a medium for special application. For example, FBS, VEGF, FGF-2, or other components may be omitted from the complete medium for specific experimental settings.   Description Size Product number Endopan 3 ready-to-use 500 ml P04-00100 Endopan 3 kit with 9 supplements 500 ml P04-0010K   Endopan MV ENDOPAN MV ready-to-use is a specially developed medium for the in vitro culture of human microvascular endothelial cells (MV-EC) containing all components necessary for optimal growth. It is designed for use in an incubator at 37° C with a 5% CO2 atmosphere. Please avoid repeated warming of complete medium. Prepare only the amount needed in a separate sterile tube. For a T25 cell culture flask it is recommended to use 5 ml of ENDOPAN MV. For smaller or larger culture area, please adjust volume accordingly.After thawing of cells, change the medium after 24 h to remove unattached cells; for maintenance and propagation, change the medium every two or three days. For cultures close to confluence or for maximum proliferative response, it is recommended to use more medium or more frequent changes. Store at 2-8° C in the dark. Expiry: 3 months. ENDOPAN MV kit is provided with FBS and supplements in separate sterile packing. This will enable the user to prepare a medium for special application. For example, FBS, VEGF, FGF-2, or other components may be omitted from the complete medium for specific experimental settings. Please note that such a formulation will not promote optimal cell growth. Therefore, this composition can not be used for routine long-term culture of endothelial cells. Please make sure that sterility is not compromised when adding individual components to prepare complete medium. The medium should be carefully but thoroughly mixed after addition of all components to assure a homogeneous solution. Store basal or complete medium at 2-8° C, store supplements at -20° C. Expiry: 12 months. Description Size Product number Endopan MV ready-to-use 500 ml P04-00200 Endopan MV kit with 8 supplements 500 ml P04-0020K     Endopan PRO Background Endothelial cells line blood and lymphatic vessels and the internal cavities of the heart. They display a strongly flattened, polygonal form and mostly rest on a basal membrane. With a total number of about 1012 cells, the endothelium is one of the biggest organs of the body and plays a key role in many physiological and pathophysiological processes. A number of factors control proliferation or apoptosis of endothelial cells, thereby regulating the maintenance, degeneration, or regeneration of blood vessels.New blood vessel formation occurs via angiogenesis or vasculogenesis, a process thought to be restricted to embryonic development. In 1997, postnatal vasculogenesis has been proposed as an important mechanism for angiogenesis via blood or bone marrow derived circulating progenitor endothelial cells (PEC) (Asahara et al, Science 1997). Consequently, PECs have been extensively studied as a potential cell therapy for the repair of damaged blood vessels. Animal studies clearly demonstrated that administration of PECs partially rescued cardiovascular dysfunction or myocardial injury with evidence for PEC contribution to new vessel growth. In most studies, PECs are defined by cell surface expression of CD34, CD133, or VEGF-R2 (KDR). Because these molecules are also present on hematopoietic progenitors, relying only on surface markers can not exclude a contamination with hematopoietic linage cells. More recently, a PEC population has been identified which shows expression of endothelial as well as progenitor, but not hematopoietic cell markers (Ingram et al, Blood. 2004;104:2752). Importantly, these cells have been tested for a high proliferative potential in clonogenic assays and additionally characterized by formation of functional blood vessels in vivo (Yoder et al, Blood. 2007;109:1801).   Composition Endopan PRO ready-to-use is a complete medium specially developed for the in vitro culture of human progenitor endothelial cells (hPEC) containing all components necessary for optimal colony formation, clonogenic growth, and rapid proliferation. Endopan PRO kit is provided with FBS growth supplement (pre-screened and tested for progenitor cells) and additional supplements in separate sterile packing. This will enable the user to prepare a medium for special application.   Description Size Product number Endopan PRO ready-to-use 500 ml P04-00700 Endopan PRO kit with 6 supplements 500 ml P04-0070K