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Electroporator for Zygote Genome Editing (Genome Editor)
Genome EditorTM is a simple and easy-to-use electroporator suitable for zygote genome editing using CRISPR/Cas9 system.

Genome Editor outputs the same pulse CUY21EDIT II outputs for zygote genome editiong.

Genome Editor performs high throughput and efficient genome editing.

Genome Editor equips an easy-to-use touch panel.
Features include:
  • High throughput and effcient CRISPR/Cas9-based mouse genome editing by RNA electroporation to zygote
  • Platinum plate electrode on tempered glass, LF501PT1-10, can hold up to ~40 mouse zygotes in a 5 μL electroporation buffer and enables efficient and high throughput zygote genome editing using CRISPR/Cas9 by electroporation.
  • More zygotes can be treated at one time by electroporation than microinjection.
  • Viability of treated zygotes are much higher in electroporation than in microinjection.
  • No special skills are required for electroporation compared to microinjection, which requires injection technique that is time-consuming to acquire.
  • Electroporation is , therefore, the ideal method for high throughput mouse zygote genome editing by CRISPR/Cas9 system.

Detail

▣ Application 


High throughput and efficient CRISPR/Cas9-based mouse genome editing by RNA electroporation to zygote.


genome_editing_result 


Fgf10 gene knockout mouse by Cas9 mRNA/gRNA electroporation

Fgf10 monozygous mutant embryo have an easily detectable limbless phenotype. Electroporation of Cas9 mRNA (Cas9 Protein) and gRNA enables genome editing of Fgf10.



◈ Zygote genome editing을 위한 Electrodes 


강화 유리에 platinum plate electrode가 장착된, LF501PT1-10은 5 uL electroportion buffer에 있는 약 40개의 mouse zygotes까지 잡을 수 있고 electroporation으로 CRISPR/Cas9을 사용하여 효율적이고 high throughput zygote genome editing을 할 수 있음


LF501PT1-10 



◈ Electroporation과 Microinjection 비교 


 

 Electroporation

 Microinjection

 Pre-operation

 Not necessary

 Preparation of injection and hold pipettes, etc.

 Required time for 100 zygotes

 ~ 5 minutes

 > 2 hours

 Viability (after E15)

 40 ~ 80%

 10 ~ 50%

 Efficiency

 Same as microinjection

 Depends on the sequence

 Required skills

 Not necessary

 Manipularion of single zygotes

 Required amount of Cas9 mRNA

 500 ~ 2,000 ng

 50 ~ 500 ng


√ 대부분의 zygotes가 microinjection보다 electroporation에 의해 한번에 처리될 수 있음 

√ 처리된 zygotes의 viability는 microinjection에서 보다 electroporation에서 훨씬 더 높음 

√ injection 기술을 익히는데 시간이 걸리는 microinjection과 비교하여, electroporation을 위한 특별한 기술이 필요 없음 

Electroporation이 CRISPR/Cas9 system으로 high throughput mouse zygote genome editing을 위한 이상적인 방법 



Order Information

Category Product Name Cat No.
Electroporator for Zygote Genome Editing   Genome Editor GEB15
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