Panserin 416 is a serum-free medium (basal medium) which is, after supplementation with growth factors, suitable for the production of dendritic cells.
Based on RPMI 1640/DMEM/F-12, trace elements, albumin, cholesterol, soya-lipids and vitamins were added to the medium. A growth factor mixture is also supplied which has to be added to the medium just before use.
Dendritic cells are highly specialized antigen-presenting cells and can initiate and regulate antigen-specific immune responses. This ability can be used in order to generate immune responses against certain proteins of tumour cells and thus the immune system itself could be able to fight against tumors. Dendritic cells have been isolated from a great variety of non-lymphatic and lymphatic tissues of human beings, mice and other species. For the generation of tumour vaccines, dendritic cells can be produced from the peripheral blood of tumor patients. In clinical studies the principal effectiveness of a vaccination with dendritic cells has been shown.