Panserin 411 is a chemically defined, complete ready-to-use medium for the serum-free cultivation of a multitude of adherent and non adherent cells which are Insulin-dependent (e.g. CHO-cells).
Based on Iscove's MEM, trace elements, albumin, cholesterol, soya lipids, vitamins and insulin were added to the medium. It does not contain any growth or attachment factors.
Panserin 411 is a multi-purpose medium suitable for a variety of cells. In Panserin 411 adherent as well as non adherent cells can be cultivated. As the medium contains no growth factors there is a possibility to investigate the effects of specific growth factors added to the cell culture. Panserin 411 does not contain any attachment factors. With some cell types a pre-treatment of the cell culture vessels with gelatine, collagen, poly-D-lysine or fibronectin may support or enable a culture under serum-free conditions. Please note that a coating may be especially important with low seeding densities.With every adaption to serum-free media, changes of the cells should be taken into consideration. These changes may concern morphology, karyotype, surface markers and so on. Thus cells in serum-free medium may not be identical with those from cultures containing serum in which they originated (selection).