Biotinylated Kinases are best suited for the study of binding affinity and other kinase-molecule interactions on devices measuring Surface Plasmon Resonance (SPR), BioLayer Interferometry (BLI) and other similar biomolecular interactions.
They can also be utilized in homogenous proximity-based binding assays such as TR-FRET to interrogate inhibitor binding affinity, determine on-off rates, and measure binding kinetics.
- Kinases are labeled with a single biotin at the N-terminus
- Easy-to use, no additional labeling required
- Native, catalytically active kinase domains are preserved
- Some kinases available either, pre-activated via ATP treatment, and without ATP pre-treatment