PowerStem HPSC is a specialized serum-free medium for the cultivation and expansion of human hematopoietic stem cells (HPSC) and cells of myeloid lineage in suspension culture. Hematopoietic stem cells are CD34+, which are the earliest hematopoietic stem cells identifiable in bone marrow, peripheral blood and neonatal cord blood. By adding one or more differentiation factors or changing culturing conditions, HPSC can be induced to differentiate into different types of hematopoietic lineage cells. PowerStem HPSC basal medium, PowerStem HPSC growth supplement and PowerStem HPSC cytokine supplement are guaranteed stable for 12 months when properly stored. PowerStem HPSC complete medium (basal + supplements) is stable for 1 month when stored in the dark at 2-8° C. We do not recommend using the complete medium beyond 1 month.
500 ml Kit
PowerStem HPSC contains purified proteins, lipids, salts, amino acids, trace elements, hormones and growth factors in an optimized formulation. PowerStem HPSC is fully chemically defined and contains no FBS or any other animal-derived components.
Serum-free cultivation and expansion of human hematopoietic CD34+ stem cells from bone marrow, peripheral blood and neonatal cord blood.
PowerStem HPSC allows the cultivation and expansion of human hematopoietic CD34+ stem cells and cells of myeloid lineage under serum-free conditions. It is fully defined in its composition and thus enables constant and comparable experimental conditions with easily reproducible results. The hematopoietic stem cells can be cultivated without stromal cells. They show a high proliferation rate and largely retain their undifferentiated state. By adding specific differentiation factors, hematopoietic cells can be differentiated in vitro to different types of hematopoietic lineage cells.